AI-Powered Fluorescence Microscopy

More Biology from Every Microscope

Sarpeda unlocks twice as many markers and pristine signal from your existing microscopes — no hardware changes, no new reagents. A pure software upgrade for the infrastructure you already own.

See It in Action
The Problem
Most microscopes leave critical biological information on the table
The photons hitting your sensors carry far more information than you're capturing. Every day, researchers force immense biological complexity through a severe bottleneck.

Limited Capacity

Just 3–4 color channels to resolve dozens of cell types, co-expression patterns, and spatial relationships. Biology is far more complex than what conventional microscopy can capture in a single acquisition.

Strong Background Noise

Autofluorescence, channel bleed-through, and non-specific binding bury the true signal. Researchers constantly fight a thick wall of noise that obscures what matters most.

The Solution
A Software Upgrade for Existing Infrastructure
Two products that sit between your microscope and downstream analysis. No hardware changes required.
2x More Markers

Sarpeda Plex

Separates two markers labeled with the same fluorophore using AI. Double your marker capacity in a single acquisition. Six markers plus DAPI on a four-channel microscope — from one tissue section with standard reagents.

Clean Signal

Sarpeda Clear

Precision removal of autofluorescence, bleed-through, and non-specific binding artifacts. A true, complete separation of all background signal — learned from routine negative controls. This is not your typical background subtraction.

See the Difference
AI-Powered Results
Drag the slider to compare input images with Sarpeda's output. The difference speaks for itself.

Sarpeda Plex — Marker Separation

Breast tissue: E-cadherin and Ki-67 labeled with the same fluorophore, then separated by AI. Green nuclei (Ki-67) clearly emerge from the red membrane staining (E-cadherin).

Breast Plex output
Breast input
Input Plex Output
Breast training image
Training image — markers imaged in separate conventional channels

Sarpeda Clear — Background Removal

Lung tissue: E-cadherin signal buried in autofluorescence, then cleaned by AI. The true biological signal emerges with single-pixel detail preserved.

Lung Clear output
Lung input
Input Clear Output
Lung negative control
Negative control — background-only reference used for AI training

Sarpeda Clear — Crosstalk Removal

Lung tissue, 568 nm channel: the target aSMA signal is contaminated by crosstalk bleeding in from the Rb in the adjacent 647 nm channel. Sarpeda Clear removes the bleed-through and recovers the true aSMA signal.

Lung crosstalk Clear output
Lung crosstalk input
Input Clear Output
Lung crosstalk negative control
Negative control — background-only reference used for AI training
Benefits
Why Sarpeda
No workflow disruption. No capital expenditure.
2x

More Markers

Six markers plus DAPI on a four-channel microscope, from a single tissue section. With indirect labeling, only three standard species needed — simpler reagents, lower cost.

Clean Signal

Precision removal of autofluorescence, channel bleed-through, and non-specific binding. Reveal the true biological signal hidden beneath noise.

Universal Fit

Works with any microscope platform. Integrates into existing analysis pipelines. No hardware changes, no new reagents. A software upgrade for existing infrastructure.

Applications
Accelerating Discovery
From diagnostics to drug discovery — anywhere fluorescence microscopy is used.

Tissue-Based Diagnostics

Conserve precious patient samples. Achieve true co-expression analysis in a single acquisition with doubled marker capacity.

Spatial Biology

More accurate RNA reporter detection with clean signal. Cut the number of imaging cycles in half.

Drug Discovery Imaging Assays

Marker-rich phenotypic assays with reliable downstream quantification. Rescue failed acquisitions that would otherwise be lost.

Organoids & Microphysiological Systems

Background removed from 3D matrices. Higher information density per sample. Fewer replicates needed.

News
Where to Find Us
September 1 to 3, 2026 · New Orleans

Sarpeda at YNOW2026, the Yokogawa Users Conference

Our CEO Ivana Mikić is giving a talk and is presenting a poster showcasing our technology. She is also participating in a panel on organoid screening scalability. The video below is looping in the exhibition hall.

October 14 to 15, 2026 · ExCeL London

Meet Sarpeda at ELRIG Drug Discovery 2026

Sarpeda is exhibiting in the Breakthrough Zone at ELRIG Drug Discovery 2026. Our AI team is at the stand with live demonstrations of marker separation and background removal.

Attendance is free. To reserve time with us at the show, get in touch.

Book a meeting at ELRIG →
Team
Leadership
Ivana Mikic

Ivana Mikić, PhD

Co-Founder & CEO

20+ years in AI and biological imaging. Scientist, inventor and repeat founder.

Advisory Board
Sinisa Todorovic

Siniša Todorović, PhD

Co-Founder & Advisor

20+ years in cutting-edge AI, spanning academia and industry.

Michael Mancini

Michael Mancini, PhD

Professor & Microscopy Core Director at Baylor College of Medicine. Early Sarpeda adopter.

Kamni Vijay

Kamni Vijay, PhD

Precision oncology and spatial biology CEO.

Lowry Curley

Lowry Curley, PhD

Principal of Luna Lifesciences. Founding CEO of AxoSim (now 28bio). Organ-on-a-chip pioneer.

Get in Touch
Let's Talk

We're signing up partners ready to transform what their microscopes can do.

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